Part:BBa_K176064:Design

aTc&AHL->PoPS: pCon 0.70->tetR+pCon 0.70->luxR+pLux/Tet->PoPS

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal NheI site found at 840
  Illegal NheI site found at 863
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1818
  

Design Notes

Input1: aTc

Input2: AHL

Output: PoPS

Design:

pCon 0.70(J23101) generates TetR(no LVA)

pCon 0.70(J23101) generates LuxR

pLux/Tet(K176000) generates PoPS, activated by LuxR and Input2 AHL, repressed by TetR and no Input1 aTc

Source

J23101 K176031